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KMID : 0881720180330040266
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Journal of Food Hygiene and Safety
2018 Volume.33 No. 4 p.266 ~ p.271
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Optimization of Analytical Methods for Octacosanol in Related Health-functional Foods with GC-MS
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Lee Jin-Hee
Oh Mi-Hyun
Lee Kyung-Jin
Kim Yang-Sun
Keum Eun-Hee
Park Ji-Eun
Cho Mee-Hyun
Seong Min-Hye
Kim Sang-A
Kim Mee-Hye
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Abstract
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The Ministry of Food and Drug Safety (MFDS) is amending its test methods for health-functional foods (dietary food supplements) to establish regulatory standards and specifications in Korea. In this regard, we continue our research on developing analytical methods for the items. Octacosanol is the major component of polycosanol and is a high-molecular-mass primary fatty alcohol, obtained from sugar cane wax. Previous researchers have shown that octacosanol can lower cholesterol and has antiaggregatory properties, cytoprotective uses, and ergogenic properties for human health. Recently, octacosanol products have been actively introduced into the domestic market because of their functional biological activity. We have developed a sensitive and selective test method for octacosanol that the TMS derivatives by means of gas-chromatographic-tandem mass spectrometry (GC-MS). The trimethylsilyl ether derivative of the target analyte showed excellent chromatographic properties. The procedure was validated in the range of 12.5~200 μg/L. Standard calibration curves presented linearity with the correlation coefficient (r2) < 0.999, and the limits of detection (LOD) and limits of quantitation (LOQ) were 4.5 μg/L and 13.8 μg/L, respectively. The high recoveries (92.5 to 108.8%) and precision (1.8 to 2.4%) obtained are in accordance with the established validation criteria. Our research can provide scientific evidence to amend the octacosanol test method for the Health-Functional Food Code.
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KEYWORD
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Octacosanol , GC-MS , Dietary supplement , Method validation
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